Inhoudsopgave
What is the morphology of Pseudomonas aeruginosa?
Pseudomonas aeruginosa is a Gram-negative, rod-shaped, asporogenous, and monoflagellated bacterium. It has a pearlescent appearance and grape-like or tortilla-like odour. P. aeruginosa grows well at 25°C to 37°C, and its ability to grow at 42°C helps distinguish it from many other Pseudomonas species.
What kingdom does Pseudomonas aeruginosa belong to?
Kingdom Monera, phylum Proteobacteria, class gamma sub-division, order Pseudomonadaceae , genus Pseudomonas , species Pseudomonas aeruginosa .
What biosafety level is Pseudomonas aeruginosa?
aeruginosa is a Biosafety Level 2 (BSL-2) pathogen. Follow your institutional guidelines for handling and safety while working with BSL-2 organisms.
Is Pseudomonas aeruginosa lactose positive?
aeruginosa isolates (beta hemolytic on blood agar; lactose-negative, oxidase-positive colonies).
Is Pseudomonas a colony morphology?
Pseudomonas produces large, opaque, flat colonies with irregular margins and distinctively fruity odour colonies. The colour of growth will depend upon the type of pigments (enumerated below) produced by the organism. The isolates from water and soil produce small round colonies.
Does Pseudomonas aeruginosa grow on pea agar?
PEA agar is a selective medium that permits the growth of gram-positive cocci while inhibiting the growth of most gram-negative organisms. aeruginosa are gram negative rods and seem to be growing equally as well as the gram positive organisms.
Is Pseudomonas aeruginosa prokaryotic or eukaryotic?
Background. The genome of Pseudomonas aeruginosa contains at least three genes encoding eukaryotic-type Ser/Thr protein kinases, one of which, ppkA, has been implicated in P. aeruginosa virulence.
Does Pseudomonas aeruginosa grow on MacConkey Agar?
Apart from the media mentioned above, Pseudomonas aeruginosa can also be grown in MacConkey agar (a bacterial culture medium commonly used to grow lactose fermenting bacteria). In MacConkey agar, Pseudomonas aeruginosa forms flat and smooth colonies that are between 2 and 3mm in diameter.
What are the 4 levels of biosafety?
Activities and projects conducted in biological laboratories are categorized by biosafety level. The four biosafety levels are BSL-1, BSL-2, BSL-3, and BSL-4, with BSL-4 being the highest (maximum) level of containment.
Is Pseudomonas aeruginosa a strict anaerobe?
P. aeruginosa is a facultative anaerobe, as it is well adapted to proliferate in conditions of partial or total oxygen depletion. This organism can achieve anaerobic growth with nitrate or nitrite as a terminal electron acceptor.
Is Pseudomonas aeruginosa lactose negative?
aeruginosa isolates (beta hemolytic on blood agar; lactose-negative, oxidase-positive colonies). Nonpigmented mucoid strains, as well as other nonpigmented organisms, will require additional testing to ensure proper identification.
Is Pseudomonas aeruginosa a biofilm?
P. aeruginosa is a well-known biofilm former, which makes it an excellent model to study biofilm formation [12,13]. A resilient biofilm is a critical weapon for P. aeruginosa to compete, survive and dominate in the cystic fibrosis lung polymicrobial environment [14].